Blood glutathione as an index of radiation-induced oxidative stress in mice and humans; PY1997; USA (美國);_IR94_

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2021-03-30
Blood glutathione as an index of radiation-induced oxidative stress in mice and humans; PY1997; USA (美國);_WJD_2021-0330_V001R01_IR94_RvD20210330_

Source (資訊來源):
https://pubmed.ncbi.nlm.nih.gov/9098094/
Info cited on 2021-03-30-WD2 (資訊引用於 中華民國110年西元2021年3月30日) by 湯偉晉 (WeiJin Tang)
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Free Radic Biol Med
. 1997;22(7):1203-9. doi: 10.1016/s0891-5849(96)00554-0.

Blood glutathione as an index of radiation-induced oxidative stress in mice and humans

J Navarro 1, E Obrador, J A Pellicer, M Aseni, J Viña, J M Estrela
PMID: 9098094 DOI: 10.1016/s0891-5849(96)00554-0

Abstract
The effect of x-rays on GSH and GSSG levels in blood was studied in mice and humans. An HPLC method that we recently developed was applied to accurately determine GSSG levels in blood. The glutathione redox status (GSH/GSSG) decreases after irradiation. This effect is mainly due to an increase in GSSG levels. Mice received single fraction radiotherapy, at total doses of 1.0 to 7.0 Gy. Changes in GSSG in mouse blood can be detected 10 min after irradiation and last for 6 h within a range of 2.0-7.0 Gy. The highest levels of GSSG (20.1 +/- 2.9 microM), a 4.7-fold increase as compared with controls) in mouse blood are found 2 h after radiation exposure (5 Gy). Breast and lung cancer patients received fractionated radiotherapy at total doses of 50.0 or 60.0 Gy, respectively. GSH/GSSG also decreases in humans in a dose-response fashion. Two reasons may explain the radiation-induced increase in blood GSSG: (a) the reaction of GSH with radiation-induced free radicals resulting in the formation of thyl radicals that react to produce GSSG; and (b) an increase of GSSG release from different organs (e.g., the liver) into the blood. Our results indicate that the glutathione redox ratio in blood can be used as an index of radiation-induced oxidative stress.

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