Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor PY2023 IR95教材

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Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor PY2023 IR95教材
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Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor PY2023 IR95教材

Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor PY2023 IR95教材

Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor PY2023 IR95教材

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2026-08-04
Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor

Source or References (資訊來源或是參考的資訊):
https://pubmed.ncbi.nlm.nih.gov/37909054/
Info cited on 2026-08-04-WD2 (資訊引用於 中華民國115年西元2026年8月4日) by 湯偉晉 (WeiJin Tang)
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Angew Chem Int Ed Engl
. 2023 Dec 18;62(51):e202313612. doi: 10.1002/anie.202313612. Epub 2023 Nov 16.

Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor


Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor (利用雙線奈米感測器於細胞內監測穀胱甘肽的抗氧化動力學)

Yu-Ting Jiao 1, Yi-Ran Kang 1, Ming-Yong Wen 1, Hui-Qian Wu 1, Xin-Wei Zhang 1, Wei-Hua Huang 1
Affiliations collapse
Affiliation
1College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
PMID: 37909054 DOI: 10.1002/anie.202313612

Abstract (摘要)

elimination
消除
elimination (消除)
消除 (elimination)

The glutathione (GSH) system is one of the most powerful intracellular antioxidant systems for the elimination of reactive oxygen species (ROS) and maintaining cellular redox homeostasis. (穀胱甘肽 (GSH) 系統是消除活性氧物質 (ROS) 及維持細胞氧化還原穩態最強大的細胞內抗氧化系統之一。)

unclear
不清楚
unclear (不清楚)
不清楚 (unclear)

remains unclear
仍然不清楚
remains unclear (仍然不清楚)
仍然不清楚 (remains unclear)

However, the rapid kinetics information (at the millisecond to the second level) during the dynamic antioxidation process of the GSH system remains unclear. (然而，在 GSH 系統動態抗氧化過程中，快速的動力學資訊 (毫秒至秒級) 仍不清楚。)

As such
因此
As such (因此)
因此 (As such)

As such, we specifically developed a novel dual-wire nanosensor (DWNS) that can selectively and synchronously measure the levels of GSH and ROS with high temporal resolution, and applied it to monitor the transient ROS generation as well as the rapid antioxidation process of the GSH system in individual cancer cells. (因此，我們特別開發了一種新型雙線奈米感測器 (DWNS)，能夠以高時間解析度選擇性且同步測量 GSH 與 ROS 的濃度，並將其應用於監測單一癌細胞內 ROS 的短暫生成以及 GSH 系統的快速抗氧化過程。)

These measurements revealed that the glutathione peroxidase (GPx) in the GSH system is rapidly initiated against ROS burst in a sub-second time scale, but the elimination process is short-lived, ending after a few seconds, while some ROS are still present in the cells. (這些測量結果顯示，GSH 系統中的穀胱甘肽過氧化物酶 (GPx) 在次秒級的時間尺度內被快速啟動以對抗 ROS 的爆發，但該消除過程短暫，在數秒後即告結束，而此時細胞內仍存在部分 ROS。)

redox imbalance-related
氧化還原失衡相關的
redox imbalance-related (氧化還原失衡相關的)
氧化還原失衡相關的 (redox imbalance-related)

This study is expected to open new perspectives for understanding the GSH antioxidant system and studying some redox imbalance-related physiological. (本研究預期將為理解 GSH 抗氧化系統以及研究某些與氧化還原失衡相關的生理機制開闢新視角。)

Abstract
The glutathione (GSH) system is one of the most powerful intracellular antioxidant systems for the elimination of reactive oxygen species (ROS) and maintaining cellular redox homeostasis. However, the rapid kinetics information (at the millisecond to the second level) during the dynamic antioxidation process of the GSH system remains unclear. As such, we specifically developed a novel dual-wire nanosensor (DWNS) that can selectively and synchronously measure the levels of GSH and ROS with high temporal resolution, and applied it to monitor the transient ROS generation as well as the rapid antioxidation process of the GSH system in individual cancer cells. These measurements revealed that the glutathione peroxidase (GPx) in the GSH system is rapidly initiated against ROS burst in a sub-second time scale, but the elimination process is short-lived, ending after a few seconds, while some ROS are still present in the cells. This study is expected to open new perspectives for understanding the GSH antioxidant system and studying some redox imbalance-related physiological.

Keywords: Amperometry; Electrochemical Nanosensor; Glutathione; Glutathione Peroxidases; Reactive Oxygen Species.

© 2023 Wiley-VCH GmbH.

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