Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis; PY2020; Wayne State University;_WJD_2024-0810_IR95_IR96_

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Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis; PY2020; Wayne State University;_WJD_2024-0810_IR95_IR96_V001R01_

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Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis; PY2020; Wayne State University;_WJD_2024-0810_IR95_IR96_V001R01_

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Maturation of cytosolic Iron-sulfur proteins requires glutathione
細胞質液 裡面的 鐵硫蛋白 的成熟需要 穀胱甘肽
Maturation of cytosolic Iron-sulfur proteins requires glutathione (細胞質液 裡面的 鐵硫蛋白 的成熟需要 穀胱甘肽)
細胞質液 裡面的 鐵硫蛋白 的成熟需要 穀胱甘肽 (Maturation of cytosolic Iron-sulfur proteins requires glutathione)

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2024-08-10
Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis

Source or References (資訊來源或是參考的資訊):
https://pubmed.ncbi.nlm.nih.gov/31672571/
Info cited on 2024-08-10-WD6 (資訊引用於 中華民國113年西元2024年8月10日) by 湯偉晉 (WeiJin Tang)
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Biochim Biophys Acta Mol Cell Biol Lipids
. 2020 Feb;1865(2):158542. doi: 10.1016/j.bbalip.2019.158542. Epub 2019 Oct 28.
Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis

Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis

Loss of the mitochondrial lipid cardiolipin leads to decreased glutathione synthesis
Vinay A Patil 1, Yiran Li 1, Jiajia Ji 1, Miriam L Greenberg 2
Affiliations collapse
Affiliations
1Department of Biological Sciences, Wayne State University, Detroit, MI, USA.
2Department of Biological Sciences, Wayne State University, Detroit, MI, USA. Electronic address: mgreenberg@wayne.edu.
PMID: 31672571 PMCID: PMC6980711 DOI: 10.1016/j.bbalip.2019.158542

Abstract
Previous studies demonstrated that loss of CL in the yeast mutant crd1Δ leads to perturbation of mitochondrial iron‑sulfur (FeS) cluster biogenesis, resulting in decreased activity of mitochondrial and cytosolic Fe-S-requiring enzymes, including aconitase and sulfite reductase. In the current study, we show that crd1Δ cells exhibit decreased levels of glutamate and cysteine and are deficient in the essential antioxidant, glutathione, a tripeptide of glutamate, cysteine, and glycine. Glutathione is the most abundant non-protein thiol essential for maintaining intracellular redox potential in almost all eukaryotes, including yeast. Consistent with glutathione deficiency, the growth defect of crd1Δ cells at elevated temperature was rescued by supplementation of glutathione or glutamate and cysteine. Sensitivity to the oxidants iron (FeSO4) and hydrogen peroxide (H2O2), was rescued by supplementation of glutathione. The decreased intracellular glutathione concentration in crd1Δ was restored by supplementation of glutamate and cysteine, but not by overexpressing YAP1, an activator of expression of glutathione biosynthetic enzymes. These findings show for the first time that CL plays a critical role in regulating intracellular glutathione metabolism.

Keywords: Barth syndrome; Cardiolipin; Fe-S cluster; Glutathione; Mitochondria; Reactive oxygen species (ROS).

Copyright © 2019 Elsevier B.V. All rights reserved.

PubMed Disclaimer

Conflict of interest statement
Declaration of competing interest

This article was prepared while Vinay A. Patil was employed at Wayne State University. The opinions expressed in this article are the author’s own and do not reflect the view of the Food and Drug Administration, the Department of Health and Human Services, or the United States Government.

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Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe; PY2023; PR China (中華人民共和國);_WJD_2024-0811_IR94 IR95_

File name:
Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe; PY2023; PR China (中華人民共和國);_WJD_2024-0811_IR94 IR95_V001R01_

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2024-08-11
Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe

Source or References (資訊來源或是參考的資訊):
https://pubs.rsc.org/en/content/articlelanding/2023/tb/d3tb00893b
Info cited on 2024-08-11-WD7 (資訊引用於 中華民國113年西元2024年8月11日) by 湯偉晉 (WeiJin Tang)
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Issue 28, 2023
Journal of Materials Chemistry B

Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe

Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe

Bioimaging of glutathione variation for early diagnosis of hepatocellular carcinoma using a liver-targeting ratiometric near-infrared fluorescent probe

Xiaoyue Han,‡a   Yanlong Xing,‡*bc   Xinyu Song,d   Kun Dou,bc   Fabiao Yu ORCID logo *bc  and  Lingxin Chen ORCID logo *a

Abstract
Reliable biomarkers are crucial for early diagnosis of diseases and precise therapy. Biological thiols (represented by glutathione, GSH) play vital roles in the antioxidant defense system for maintaining intracellular redox homeostasis in organisms. However, the aberrant variation in the cellular concentration of GSH correlates with diverse diseases including cancer. Here, a ratiometric near-infrared fluorescent probe CyO-Disu is constructed for the specific sensing of GSH variation in live cells and mice models of hepatic carcinoma (HCC). CyO-Disu features three key elements, a response moiety of bis(2-hydroxyethyl) disulfide, a near-infrared fluorescence signal transducer of heptamethine ketone cyanine, and a targeting moiety of D-galactose. By virtue of its liver-targeting capability, CyO-Disu was utilized for evaluating GSH fluctuations in primary and metastatic hepatoma living cells. To evaluate the efficacy of CyO-Disuin vivo, orthotopic HCC and pulmonary metastatic hepatoma mice models were employed for GSH imaging using two-dimensional and three-dimensional fluorescence molecular tomographic imaging systems. The bioimaging results offered direct evidence that GSH displayed varied concentrations during the progression of HCC. Therefore, the as-synthesized probe CyO-Disu could serve as a potential powerful tool for the early diagnosis and precise treatment of HCC using GSH as a reliable biomarker.

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Effect of oxidized and reduced glutathione in liver preservation PY1990 IR95 肝臟的保存

資訊來源：

https://pubmed.ncbi.nlm.nih.gov/2256168/

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December 1990

Effect of oxidized and reduced glutathione in liver preservation

K Boudjema 1, T M Van Gulik, S L Lindell, P S Vreugdenhil, J H Southard, F O Belzer

Affiliation

Department of Surgery, University of Wisconsin, Madison 53792.

PMID: 2256168

Abstract

Glutathione spontaneously oxidizes in the UW solution for organ preservation and reduced glutathione (GSH) is converted to oxidized (GSSG) glutathione. To determine the effects of the oxidized or reduced forms of glutathione on liver preservation dog livers were preserved for 24 and 48 hr with the UW solution containing either GSH or GSSG. After 24 hr of preservation the form of glutathione did not affect survival or the postoperative course of the animals. All animals survived (three per group) with near-normal liver functions by the third to fifth postoperative day. When preservation was extended to 48 hr survival was 100% (6/6) with GSH and 29% (2/7) with GSSG. The dogs that died developed primary nonfunction of the liver. This study shows that GSH is an important component of the UW solution for 48-hr preservation of the dog liver. The presence of GSSG does not prevent successful 24-hr preservation of the liver, which has been confirmed in clinical studies. However, for 48-hr preservation GSH is required and GSSG is not suitable.

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Effect of oxidized and reduced glutathione in liver preservation PY1990 IR95 肝臟的保存

Effect of oxidized and reduced glutathione in liver preservation PY1990 IR95 肝臟的保存

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Plasma Glutathione Levels Decreased with Cognitive Decline among People with Mild Cognitive Impairment (MCI): A Two-Year Prospective Study PY2021 IR95 IR96 Taiwan

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2024-05-31
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Plasma Glutathione Levels Decreased with Cognitive Decline among People with Mild Cognitive Impairment (MCI): A Two-Year Prospective Study PY2021 IR95 IR96 Taiwan

Plasma Glutathione Levels Decreased with Cognitive Decline among People with Mild Cognitive Impairment (MCI): A Two-Year Prospective Study PY2021 IR95 IR96 Taiwan
在 輕度認知障礙的患者 中，血漿裡面的 穀胱甘肽 之水平 隨著 認知能力 的下降而逐漸減少：一個為期兩年的前瞻性研究計畫

資訊來源 2024-0522：
https://pubmed.ncbi.nlm.nih.gov/34829710/

#湯偉晉挑選的醫學論文

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Antioxidants (Basel)

2021 Nov 19;10(11):1839. doi: 10.3390/antiox10111839.
Plasma Glutathione Levels Decreased with Cognitive Decline among People with Mild Cognitive Impairment (MCI): A Two-Year Prospective Study
Chieh-Hsin Lin 1 2 3, Hsien-Yuan Lane 3 4 5

Affiliations collapse
Affiliations
1
Department of Psychiatry, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan. 長庚大學醫學院高雄長庚醫院精神科，台灣高雄
2
School of Medicine, Chang Gung University, Taoyuan 33302, Taiwan 長庚大學醫學院, 台灣 桃園
3
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan. 中國醫藥大學，台中
4
Department of Psychiatry & Brain Disease Research Center, China Medical University Hospital, Taichung 40402, Taiwan.
5
Department of Psychology, College of Medical and Health Sciences, Asia University, Taichung 41354, Taiwan.

PMID: 34829710
PMCID: PMC8615204
DOI: 10.3390/antiox10111839

Abstract
Glutathione (GSH) is a major endogenous antioxidant. Several studies have shown GSH redox imbalance and altered GSH levels in Alzheimer's disease (AD) patients. Early detection is crucial for the outcome of AD. However, whether GSH can serve as a biomarker during the very early-phase of AD, such as mild cognitive impairment (MCI), remains unknown. The current prospective study aimed to examine the longitudinal change in plasma GSH concentration and its influence on cognitive decline in MCI. Overall, 49 patients with MCI and 16 healthy individuals were recruited. Plasma GSH levels and cognitive function, measured by the Mini-Mental Status Examination (MMSE) and Alzheimer's disease assessment scale-cognitive subscale (ADAS-cog), were monitored every 6 months. We employed multiple regressions to examine the role of GSH level in cognitive decline in the 2 years period. The MCI patients showed significant decline in plasma GSH levels and cognitive function from baseline to endpoint (month 24). In comparison, the healthy individuals' GSH concentration and cognitive function did not change significantly. Further, both GSH level at baseline and GSH level change from baseline to endpoint significantly influenced cognitive decline among the MCI patients. To our knowledge, this is the first study to demonstrate that both plasma GSH levels and cognitive function declined 2 years later among the MCI patients in a prospective manner. If replicated by future studies, blood GSH concentration may be regarded as a biomarker for monitoring cognitive change in MCI.

 

 

Begin_電腦自動翻譯的內容_Y2024M05D31H03M27Rn3488_
(請注意：電腦自動翻譯的內容，可能會夾雜一些錯誤！)
穀胱甘肽 (GSH) 是一種主要的內源性抗氧化劑。多項研究表明，阿茲海默症 (AD) 患者存在 GSH 氧化還原失衡和 GSH 水平改變。早期發現對於 AD 的結果至關重要。然而，GSH 是否可以作為 AD 早期階段（例如輕度認知障礙（MCI））的生物標記仍不清楚。目前的前瞻性研究旨在探討血漿 GSH 濃度的縱向變化及其對 MCI 認知能力下降的影響。總體而言，招募了 49 名 MCI 患者和 16 名健康個體。每 6 個月監測血漿 GSH 水平和認知功能，並透過簡易精神狀態檢查 (MMSE) 和阿茲海默症評估量表-認知分量表 (ADAS-cog) 進行測量。我們採用多元迴歸來檢驗 GSH 水準在兩年內認知能力下降中的作用。 MCI 患者的血漿 GSH 水平和認知功能從基線到終點（第 24 個月）顯著下降。相較之下，健康個體的GSH濃度和認知功能則沒有顯著變化。此外，基線時的 GSH 水平以及從基線到終點的 GSH 水平變化均顯著影響 MCI 患者的認知能力下降。據我們所知，這是第一個前瞻性地證明 MCI 患者血漿 GSH 水平和認知功能在 2 年後下降的研究。如果未來的研究能夠重複，血液 GSH 濃度可能被視為監測 MCI 認知變化的生物標記。
End_電腦自動翻譯的內容_Y2024M05D31H03M27Rn3488_

Keywords: Alzheimer’s disease (AD); biomarker; cognitive function; glutathione (GSH); mild cognitive impairment (MCI).
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Conflict of interest statement
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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