L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential; PY2013; India (印度);_WJD_2023-0213_IR95_

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L-cysteine efflux in erythrocytes as a function of human age： correlation with reduced glutathione and total anti-oxidant potential; PY2013; India (印度);_WJD_2023-0213_IR95_.docx
L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential; PY2013; India (印度);_WJD_2023-0213_IR95_

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2023-02-13
L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential; PY2013; India (印度);_WJD_2023-0213_IR95_
Source or References (資訊來源或是參考的資訊):
https://pubmed.ncbi.nlm.nih.gov/23442131/
Info cited on 2023-02-13-WD1 (資訊引用於 中華民國112年西元2023年2月13日) by 湯偉晉 (WeiJin Tang)
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Rejuvenation Res
. 2013 Jun;16(3):179-84. doi: 10.1089/rej.2012.1394.
L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential

L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential

L-cysteine efflux in erythrocytes as a function of human age: correlation with reduced glutathione and total anti-oxidant potential
Prabhanshu Kumar 1, Pawan Kumar Maurya
Affiliations collapse
Affiliation
1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India.
PMID: 23442131 DOI: 10.1089/rej.2012.1394
Abstract
Thiol compounds such as cysteine (Cys) and reduced glutathione (GSH) play an important role in human aging and age-related diseases. In erythrocytes, GSH is synthesized by glutamic acid, cysteine, and glycine, but the rate of GSH synthesis is determined only by the availability of L-cysteine. Cysteine supplementation has been shown to ameliorate (改善，使變好) several parameters that are known to degenerate during human aging. We have studied L-cysteine efflux in vitro in human erythrocytes as a function of age by suspending cells in solution containing 10 mM L-cysteine for uptake; later cells were re-suspended in phosphate-buffered saline (PBS)-glucose to allow efflux. Change in the free sulfhydryl (-SH) concentration was then measured to calculate the rate of efflux. The GSH/oxidized glutathione (GSSG) ratio was taken as a control to study the oxidation/reduction state of the erythrocyte. The total anti-oxidant potential of plasma was measured in terms of ferric reducing ability of plasma (FRAP) values. We have shown a significant (p<0.0001) decline in the efflux of L-cysteine in erythrocytes during human aging, and the GSH/GSSG ratio decreases as a function of human age. The decline in L-cysteine efflux during aging correlates with the decrease in GSH and the FRAP value. This finding may help to explain the shift in the redox status and low GSH concentration that might determine the rate of L-cysteine efflux observed in erythrocytes and an important factor in the development of oxidative stress in erythrocytes during aging.

 

 

Begin_電腦自動翻譯的內容_Y2023M02D13H08M25Rn8880_
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半胱胺酸 (Cys) 和還原型穀胱甘肽 (GSH) 等硫醇化合物在人類衰老和與年齡相關的疾病中起著重要作用。在紅血球中，GSH 由谷胺酸、半胱胺酸和甘胺酸合成，但 GSH 合成速率僅由 L-半胱胺酸的可用性決定。已證明補充半胱胺酸可以改善已知在人類衰老過程中退化的幾個參數。我們通過將細胞懸浮在含有 10 mM L-半胱胺酸的溶液中進行吸收，研究了人紅血球體外 L-半胱胺酸流出與年齡的關係； 後來的細胞被重新懸浮在磷酸鹽緩衝鹽水（PBS）-葡萄糖中以允許流出。然後測量游離巰基 (-SH) 濃度的變化以計算流出率。GSH/氧化型穀胱甘肽 (GSSG) 比率作為對照來研究紅血球的氧化/還原狀態。根據血漿鐵還原能力 (FRAP) 值測量血漿的總抗氧化潛力。我們已經表明，在人類衰老過程中，紅血球中 L-半胱胺酸的流出顯著 (p<0.0001) 下降，並且 GSH/GSSG 比率隨著人類年齡的增長而下降。衰老過程中 L-半胱胺酸外流的下降與 GSH 和 FRAP 值的下降相關。這一發現可能有助於解釋氧化還原狀態的變化和低穀胱甘肽濃度，這可能決定了在紅血球中觀察到的 L-半胱胺酸流出率，並且是紅血球在衰老過程中氧化壓力發展的重要因素。
End_電腦自動翻譯的內容_Y2023M02D13H08M25Rn8880_

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