Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry; glutathione; PY2018; Japan (日本);_IR95_

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2021-03-30
Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry; glutathione; PY2018; Japan (日本);_WJD_2021-0330_V001R01_IR95_RvD20210330_

Source (資訊來源):
https://pubmed.ncbi.nlm.nih.gov/29743580/
Info cited on 2021-03-30-WD2 (資訊引用於 中華民國110年西元2021年3月30日) by 湯偉晉 (WeiJin Tang)
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2018 May 9;8(1):7425. doi: 10.1038/s41598-018-25650-y.

Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry

Lue Sun 1 2, Yohei Inaba 3 4, Keizo Sato 5, Aki Hirayama 6, Koji Tsuboi 1, Ryuji Okazaki 2, Koichi Chida 3 4, Takashi Moritake 7

Affiliations
1Department of Radiation Biology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-0006, Japan.
2Department of Radiological Health Science, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan.
3Course of Radiological Technology, Health Sciences, Tohoku University Graduate School of Medicine, 2-1 Seiryo, Aoba, Sendai, Miyagi, 980-8575, Japan.
4Department of Radiation Disaster Medicine, International Research Institute of Disaster Science, Tohoku University, Aramaki Aza-Aoba 468-1, Aoba-ku, Sendai, 980-0845, Japan.
5School of Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino Nobeoka, Miyazaki, 882-8508, Japan.
6Center for Integrative Medicine, Tsukuba University of Technology, Kasuga 4-12-7, Tsukuba, 305-8521, Japan.
7Department of Radiological Health Science, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan. moritake@med.uoeh-u.ac.jp.

PMID: 29743580 PMCID: PMC5943295 DOI: 10.1038/s41598-018-25650-y

Abstract
Many reports have demonstrated that radiation stimulates reactive oxygen species (ROS) production by mitochondria for a few hours to a few days after irradiation. However, these studies were performed using cell lines, and there is a lack of information about redox homeostasis in irradiated animals and humans. Blood redox homeostasis reflects the body condition well and can be used as a diagnostic marker. However, most redox homeostasis studies have focused on plasma or serum, and the anti-oxidant capacity of whole blood has scarcely been investigated. Here, we report changes in the anti-oxidant capacity of whole blood after X-ray irradiation using C57BL/6 J mice. Whole-blood anti-oxidant capacity was measured by electron spin resonance (ESR) spin trapping using a novel spin-trapping agent, 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO). We found that whole-blood anti-oxidant capacity decreased in a dose-dependent manner (correlation factor, r > 0.9; P < 0.05) from 2 to 24 days after irradiation with 0.5-3 Gy. We further found that the red blood cell (RBC) glutathione level decreased and lipid peroxidation level increased in a dose-dependent manner from 2 to 6 days after irradiation. These findings suggest that blood redox state may be a useful biomarker for estimating exposure doses during nuclear and/or radiation accidents.

Conflict of interest statement
The authors declare no competing interests.

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